Migration assay of urothelial cancer cells after HDAC8 inhibitor treatment. (A) Representative photographs of wound healing assay at 0 and 12 hours from inhibitor treated RT-112, VM-CUB1, SW-1710, 639-V and UM-UC-3 cells (compound 2, compound 5, compound 6; IC50, 72 h) in comparison to a DMSO solvent control (co). (B) Relative scratch size after 3, 6, 9 and 12 h of migration in comparison to the starting point 0 h. The relative scratch size is displayed on the y-axis. p < 0.05 was regarded as significant and marked as *, whereas p < 0.01 and p < 0.001 were defined as highly significant and marked as ** and ***. The calculated significances refer to the DMSO solvent control.