PLAG1 is essential for the role of KPNA2 in proliferation and invasion of tumor cells. (a-b) The cell proliferation of HCC cells was assayed every 12 hours for two days in three independent experiments. ★ represents statistical significance compared to Scramble or GFP cells. (c-d) The number of migratory HCC cells was calculated with crystal violet staining and representative fields were exhibited. Bar graphs in left panel show mean the average count of six random microscopic fields and the mean SEM. ★ represents statistical significance.