Table 1 Validation of MS-MLPA results for ATM, MLH1 and FHIT
Gene | Method | Primer sequence/polyclonal antibody | No. samples examined | Overall concordance (%) |
|---|---|---|---|---|
ATM | Pyrosequencing CpG analysis | Fw: 5′-AGAAGTGGGAGTTGGGTAGTT-3′ | 77/78 | 73% |
Rv: 5′-biotinCTCCCCCCCCCTACCACTACACTC-3′ | ||||
Seq: 5′-AGGAGGAGAGAGGAGT-3′ | ||||
MLH1 | Pyrosequencing CpG analysis | Fw: 5′-biotinGGGAGGTAAGTTTAAGTGGAATAT-3′ | 72/78 | 79% |
Rv: 5′-CCAATCCCCACCCTAAAACCCTC-3′ | ||||
Seq: 5′-CTAAACTCCCAAATAATAACCT-3′ | ||||
FHIT | Immunohistochemistry | Rabbit polyclonal anti-FHIT; clone PA1-37690; Thermo Scientific Pierce; working dilution: 1/200 | 57/78 | 84% |