Table 1 Sequence of primers used in qRT-PCR
From: miR-19a acts as an oncogenic microRNA and is up-regulated in bladder cancer
Primer | Sequence (5’ → 3’) |
|---|---|
miR-19a-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAGTTT |
miR-19a-forward | CTGGAGTGTGCAAATCTATGC |
miR-19a-reverse | GTGCAGGGTCCGAGGT |
miR-19a-probe | FAM-CTGGATACGACTCAGTTT-MGB |
U6-RT | AAAATATGGAACGCTTCACGAATTTG |
U6-forward | CTCGCTTCGGCAGCACATATACT |
U6-reverse | ACGCTTCACGAATTTGCGTGTC |
U6-probe | FAM-CCATGCTAATCTTCTCTGTA-MGB |