Skip to main content
Figure 3 | Journal of Experimental & Clinical Cancer Research

Figure 3

From: MicroRNA-320c inhibits tumorous behaviors of bladder cancer by targeting Cyclin-dependent kinase 6

Figure 3

CDK6 is a direct target of miR-320c. (A) An increased expression pattern of CDK6 was observed in UM-UC-3 and T24 cells compared with SV-HUC-1 cells. (B, C) Over-expression of miR-320c reduced CDK6 expression level in both cell lines significantly (levels of mRNA and protein). (D) A predicted seed region in the 3′-UTR of CDK6 was illustratred (top). The mutated sequence was highlighted in underline (bottom). (E) 293 T cells were co-transfected with 50nM of either miR-320c mimic or NC oligos and 200 ng plasmid containing Wt or Mut of CDK6 3’-UTR. The relative firefly luciferase activity normalized with Renilla luciferase was calculated 48 h after transfection (*P < 0.05).

Back to article page