Figure 3

Influence of PARs on cleavage reaction. Suicide cleavage reaction as a function of PAR concentration, carried out with the substrate described on the top of the figure. Different concentrations of PARs were incubated with the wild-type or 8bmut proteins at 37°C for 30 min. Cl represents the DNA fragment cleaved by the enzyme at the preferred site. Lane 1, no protein added. The percentage of cleaved suicide substrate has been plotted against different PAR concentrations for the wild-type (dark grey bar) and the 8bmut (light gray bar). Data shown are means ± SD from 3 independent experiments.