The cell early apoptotis rate was detecte by flow cytometry. LNCaP and RWPE-1 cells cells were serum-starved for 6 h and then transfected with siRNA PCGEM1 or PmiR-145. After 48 h cells were collected and fixed in paraformaldehyde and ethanol overnight. Cells were stained by propidium iodide (PI) and with FITC-conjugated AnnexinV, analyzed by flow cytometry with a BD FACScalibur (BD Bioscience, Heidelberg, Germany). Mean values of three independent experiments with standard errors are represented. A: Representative Annexin V/PI double staining LNCaP cells. B: Representative Annexin V/PI double staining RWPE-1 cells. C: Comparison of the percentage of early apoptosis in LNCaP cells, P△* < 0.05 vs control and NC group. D: Comparison of the percentage of early apoptosis in RWPE-1 cells, P△* > 0.05 vs control and NC group. 1: Control group; 2: Negative group: 3: siRNA PCGEM1 group. 4. PmiR-145 group.