Figure 5

SPOP promotes tumor cell apoptosis. (A) Overexpression of SPOP upregulates the expression of apoptotic proteins. MKN45 cells were transfected with Myc-SPOP and incubated for 48 h. Target proteins in cell lysates were detected by indicated antibodies using Western blotting. (B) Repression of SPOP inhibits cancer cell apoptosis. MKN45 cells were transfected with miR-SPOP-1430 and incubated for 48 h. Target proteins in cell lysates were probed with indicated antibodies using Western blotting. (C) Overexpressed SPOP promotes cleaved Caspase-3 expression. AGS cells were transfected with Myc-SPOP for 48 h and then fixed. Myc-SPOP was detected by incubating cells with mouse anti-Myc antibody and subsequently Alexa Fluor 488 goat anti-mouse antibody. Cleaved Caspase-3 was detected by incubating cells with rabbit anti-cleaved Caspase-3 and subsequently Alexa Fluor 594 donkey anti-rabbit antibody. Nucleus was identified by DAPI staining. (D) The representative photos of cleaved Caspase-3 expression in gastric cancer tissues and adjacent gastric tissues by using immunohistochemical staining (DAB staining, scale bar, 100 μm). Magnified local images reflecting detailed information were shown on the bottom.