Decreased expression level of MICA/B on hepatoma cells following tunicamycin(TM) treatment. (A) Surface expression level of MICA/B on SMMC7721 and HepG2 cells was analyzed using flow cytometry. The grey histograms represent the isotype control staining. (B) Upper panel: data on the y-axes represent the mean percentage positive staining. Lower panel: data on the y-axes represent the mean florescence intensity. Data were presented as means ± S.D., *P < 0.05, **P < 0.01. (C) Total protein expression level of MICA/B in SMMC7721 and HepG2 cells treated with DMSO or TM for 16 or 24 h were analyzed using a western blot assay. GAPDH was detected as a loading control. The data shown are representative of three individual experiments.