Figure 1

High glucose (HG) culture condition reduces the cytotoxic effects of drugs in wtp53-carrying tumor cells. (A) Cells were plated at subconfluence in culture media containing 10% FBS and 1 g/L D-glucose. The day after, medium was changed with a medium containing 2% FBS with either 1 g/L D-glucose or 4.5 g/L D-glucose, respectively low and high glucose (HG), for 24 h before adding chemotherapeutic drugs Adriamycin (ADR) (to RKO, HCT116 and HCT116-p53^-/- cells) at 2 μg/ml or cisplatin (CDDP) (to A549 and 2008 cells) at 5 μg/ml. Twenty-four hours later, the percentage of dead cells was scored by trypan blue staining. Error bars show standard deviation. *P=0.002. (B) HCT116 cells were treated with ADR (2 μg/ml) with or without HG culture condition. Equal amount of total cell extracts was analysed by western immunoblotting with anti-PARP antibody specific for the cleaved form (cleav). Anti-β-actin was used as protein loading control.