Figure 3
From: CD74 interferes with the expression of fas receptor on the surface of lymphoma cells
Over-expression of CD74 protects mice from a lethal dose of agonistic Fas antibody. Mice were transfected with an empty plasmid (vector) or plasmid encoding Myc-tagged CD74 using a hydrodynamic transfection method. Twenty-four hours post transfection, mice were challenged with a lethal dose of agonistic anti-Fas antibody Jo2 and monitored for survival up to 6 hours. (A) Survival of challenged mice at 6 hours; (B) gross appearance of livers from Jo2 challenged mice; (C) immunohistochemistry of liver tissues from control (vector) and Jo2-challenged (vector with Jo2 and CD74-Myc with Jo2) mice. H&E - hematoxylin and eosin staining, MYC - Myc-tag staining, Cleaved Caspase 3 - apoptosis staining.