Table 2 Methods for the detection of immunohistochemical expression of the tested markers
| Â | Company | Antigen | Blocking | 1.AK | 2.AK |
|---|---|---|---|---|---|
retrieval | 30’RT | ||||
AREG | R&D Systems | 0,1 M | 5% swine | 1:75, | LSAB + System-HRP |
| Â | AF262 | EDTA buffer pH8 | serum | over night | K 0690 |
EREG | R&D Systems | - | 5% swine | 1:100 | LSAB + System-HRP |
| Â | AF1195 | Â | serum | over night | K 0690 |
VEGF-C | Sant Cruz | - | FFP | 1:50 | Dako Realâ„¢EnVisionâ„¢Detection System |
| Â | sc-9047 | Â | Â | over night | K 5007 |
VEGF-D | Sant Cruz | 0.01 M | FFP | 1:50 | Dako Realâ„¢EnVisionâ„¢Detection System |
| Â | sc-13085 | Citrat buffer | Â | 2 h RT | K 5007 |
VEGFR-3 | Sant Cruz | 0.01 M | FFP | 1:100 | LSAB + System-HRP |
| Â | sc-321 | Citrat buffer | Â | 2 h RT | K 0690 |
Hif-1 α | Sant Cruz | 0,1 M | 5% swine | 1:50 | LSAB + System-HRP |
| Â | sc-53546 | EDTA buffer pH8 | serum | over night | K 0690 |
PTEN | Cell Signaling | 0.01 M | 5% goat | 1:75 | Dako Realâ„¢EnVisionâ„¢Detection System |
|  | 9188’s | Citrat buffer | serum | over night | K 5007 |