| Company | Antigen | Blocking | 1.AK | 2.AK |
---|---|---|---|---|---|
retrieval | 30’RT | ||||
AREG | R&D Systems | 0,1 M | 5% swine | 1:75, | LSAB + System-HRP |
 | AF262 | EDTA buffer pH8 | serum | over night | K 0690 |
EREG | R&D Systems | - | 5% swine | 1:100 | LSAB + System-HRP |
 | AF1195 |  | serum | over night | K 0690 |
VEGF-C | Sant Cruz | - | FFP | 1:50 | Dako Realâ„¢EnVisionâ„¢Detection System |
 | sc-9047 |  |  | over night | K 5007 |
VEGF-D | Sant Cruz | 0.01 M | FFP | 1:50 | Dako Realâ„¢EnVisionâ„¢Detection System |
 | sc-13085 | Citrat buffer |  | 2 h RT | K 5007 |
VEGFR-3 | Sant Cruz | 0.01 M | FFP | 1:100 | LSAB + System-HRP |
 | sc-321 | Citrat buffer |  | 2 h RT | K 0690 |
Hif-1 α | Sant Cruz | 0,1 M | 5% swine | 1:50 | LSAB + System-HRP |
 | sc-53546 | EDTA buffer pH8 | serum | over night | K 0690 |
PTEN | Cell Signaling | 0.01 M | 5% goat | 1:75 | Dako Realâ„¢EnVisionâ„¢Detection System |
 | 9188’s | Citrat buffer | serum | over night | K 5007 |