Figure 1

Cancer patient serum and cancer cell line conditioned medium increased HEK293 cells growth. HEK293 cells were cultured for 3 weeks in control human serum, or cancer patient sera (A-C), and in control medium or Colo320 cell line conditioned medium (CM-Colo) (D-F). Cells were than analyzed for their growth potential. (A and D) population doublings capability was calculated at every passage. Column graphs represent cumulative population doublings at the end of the treatment periods. (B and E) metabolic activity following 6 hours incubation with Alamar Blue and spectrofluorometry analyses. (C and F) proliferation following labeling with CFSE probe and cytometry acquisition. Numbers in brackets are the mean fluorescence intensity (MFI) of each peak. Data are mean × SD of 2 control sera vs. 4 cancer patient sera (A-C), and 2 control vs. 3 independent batches of conditioned media (D-F). (See Additional file 2: Figure S1 for supplement information)