Figure 3

Effect of cancer patient serum and cancer cell line conditioned medium on tumorigenicity of HEK293 cells in vivo. SCID/Beige mice were injected with HEK293 cells cultured for 3 weeks in control human serum, or cancer patient sera, and in control medium or Colo320 cell line conditioned medium (CM-Colo). (A) 4 to 5 weeks after injection, mice were photographed and euthanized. Representative pictures of tumors are shown. (B and C) tumor growth was monitored weekly. Once tumors were palpable, their diameters were measured (B) and their volumes at euthanasia were calculated (C). Values are mean +/− SD, (n = 3−6 mice per group). (D) Formalin-fixed paraffin-embedded xenotransplant samples were processed for H&E staining (i), and immunolabeled with antibodies against (ii) cytokeratin or (iii) vimentin (brown). Scale bars, 50 μm. (See Additional file 5: Figure S4 for supplement information)