Figure 1

Methylation analyses of PDSS2 in GC cell lines. (A) The CpG island indicated by the blue line is centered on the PDSS2 transcription initiation site extending upstream into the promoter region. (B) Bar graphs indicate PDSS2 mRNA expression levels in CCL-241 (control non-cancerous cell) and GC cell lines before or after 5-aza-dC treatment. The methylation status of the PDSS2 promoter was evaluated using MSP, and the results are enclosed in the box. M, methylated; pM, partially methylated; U, unmethylated. (C) Representative results of bisulfite sequence analysis. All CpG sites in KATOIII cell were retained as CG and those of MKN74 were converted to TG.