SKF96365 inhibited focal adhesion turnover, EMT-like, and Pyk2 phosphorylation in U251 and SNB19 glioma cells. (A and B) Immunofluorescent staining for vinculin showed differences in focal adhesions between the control and SKF96365-treated glioma cells. Magnification, ×1000. The proportions of cells with large focal adhesions are presented as means ± SDs of nine different microscopic visions from three independent experiments. **P < 0.01. (C) Western blot analysis of E-cadherin, N-cadherin, and vimentin in U251 and SNB19 cells treated with SKF96365. (D) Western blot analysis of the total Pyk2 and p-Pyk2 in U251 and SNB19 cells after treatment with SKF96365. The gray values represent the means of three independent experiments (normalized to GAPDH expression).