Figure 4

Orai1 controlled U251 and SNB19 cell invasion through regulation of Pyk2 activity. (A) The expression of Orai1 in U251 and SNB19 cells transfected with shControl, shOrai1, or Orai1 rescue by western blot analysis. (B) Changes in Ca²⁺ influx in U251 and SNB19 cells transfected with shControl, shOrai1, or Orai1 rescue according to Fluo-4 Ca²⁺ measurements. Each trace was obtained by averaging data from 25–45 single cells. TG, thapsigargin. (C) Western blot analysis of the total Pyk2 and p-Pyk2 in U251 and SNB19 cells transfected with shControl, shOrai1, Orai1 rescue, or shPyk2. (D) Invasive ability of U251 and SNB19 cells transfected with shControl, shOrai1, Orai1 rescue, or shPyk2 as examined by Matrigel-coated Boyden chamber assays. Data are presented as means ± SDs of nine different microscopic visions from three independent experiments. **P < 0.01.