Figure 2

Enforced expression of miR-133b suppresses cell proliferation and colony formation ability of GC cells. (A) Overexpression of miR-133b in HGC-27 cells was confirmed by qRT-PCR. (B) The cell growth of HGC-27 cells at day 0, 1, 2, 3, 4 post transfection which was detected by CCK-8 assay. Data are shown as mean ± s.d. (n = 3); ** indicates P-value <0.01. (C) Overexpression of miR-133b in MGC-803 cells was confirmed by qRT-PCR. (D) The cell growth of MGC-803 cells at day 0, 1, 2, 3, 4 post transfection which was detected by CCK-8 assay. Data are shown as mean ± s.d. (n = 3); ** indicates P-value <0.01; *** indicates P-value <0.001. (E) The colony number of HGC-27 cells per well in 6-well plates cultured for 7 days. (F) The colony number of MGC-803 cells per well in 6-well plates cultured for 7 days.