Figure 5

FSCN1 is a direct target of miR-133b in GC cells. (A) Schematic representation of FSCN1 3′ UTR showing putative miR-133b binding site. (B) Relative luciferase activity of FSCN1 3′ UTR reporter constructs in 293 T cells. Data are shown as mean + s.d. (n = 3); * indicates P-value <0.05, ** indicates P-value <0.01. (C) Relative luciferase activity of wild type and mutated FSCN1 3′ UTR constructs in 293 T cells. Data are shown as mean + s.d. (n = 3); * indicates P-value <0.05, ** indicates P-value <0.01. (D) Western blot analysis of FSCN1 expression in HGC-27 and MGC-803 cells transfected with scramble control or miR-133b mimics; FSCN1 expression level in GES, HGC-27 and MGC-803 cells.