Table 1 Sequence of primers used in qRT-PCR and constructs
From: The role of microRNA-133b and its target gene FSCN1 in gastric cancer
Primer | Sequence (5′ → 3′) |
|---|---|
miR-133b-RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGCAGG |
miR-133b-forward | CTGGAGTTTGGTCCCCTTCAAC |
miR-133b-reverse | GTGCAGGGTCCGAGGT |
miR-133b-probe | FAM-ATACGACTAGCAGGTTGA-MGB |
U6-RT | AAAATATGGAACGCTTCACGAATTTG |
U6-forward | CTCGCTTCGGCAGCACATATACT |
U6-reverse | ACGCTTCACGAATTTGCGTGTC |
U6-probe | FAM-CCATGCTAATCTTCTCTGTA-MGB |
FSCN1-UTR-F | TGGGCTAGGACTGACCCTTGT |
FSCN1-UTR-R | GGGAGCACCAATCACAGCA |
FSCN1-UTR-Mut Sense | GAAAATCGCTCATTCAGTATTT |
FSCN1-UTR-Mut Anti | TACTGAATGAGCGATTTTCTGCTT |