PTHLH mRNA and PTHrP protein expression in OSCC specimens and cell lines. (A) PTHLH mRNA expression was analyzed in 36 paired OSCC specimens including cancerous tissues and apparently normal surgical margins using real-time PCR (Mean ± SE: 2.618 ± 0.495, rang: 0.0817 ± 12.010 vs. Mean ± SE: 7.376 ± 1.040, rang: 0.988 ± 20.873; P = 0.0001; two-tailed Student’s t-test). (B) PTHrP protein expression was determined in 5 representative paired OSCC specimens by western blotting analysis (N, apparently normal surgical margin; C, cancerous tissue). (C) PTHLH mRNA levels were determined in 9 representative OSCC cell lines, primary normal epithelial cells and normal tissues showing in Figure 1A using real-time PCR (P < 0.0001; One-way ANOVA). (D) PTHrP protein levels were determined in 9 representative OSCC cell lines and primary normal epithelial cells using western blotting.