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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Parathyroid hormone-related protein serves as a prognostic indicator in oral squamous cell carcinoma

Figure 2

The effect of PHTLH/PTHrP on cell proliferation and colony formation. (A) PTHrP protein expression was confirmed in WSU-HN6, HN13 and CAL-27 cell lines transfected with anti-PTHLH siRNA P-si or scrambled siRNA using western blotting. (B) Cell proliferation of WSU-HN6, HN13 and CAL-27 cell lines transfected with anti-PTHLH siRNA P-si or scrambled siRNA were analyzed using the CCK-8 kit, each point showed the mean values of triplicate wells (Mean ± SE). (C) Plate colony formation of WSU-HN6, HN13 and CAL-27 cell lines transfected with anti-PTHLH siRNA P-si or scrambled siRNA was analyzed. (D) Soft agar colony formation assay was analyzed between the cell lines transfected with anti-PTHLH P-si and cell lines transfected with scrambled siRNA.

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