Figure 2

Interaction of PEG-ADI and gemcitabine. A) Dose-dependent effect of combining gemcitabine with PEG-ADI in MIA-PaCa-2 cells determined by MTT cytotoxicity assay, B) Effect of PEG-ADI, gemcitabine, or the combination on cell death measured by flow cytometry in MIA-PaCa-2 (mean of three separate experiments, +/- S.D), C) Effect of PEG-ADI, gemcitabine, or the combination on cell death measured by flow cytometry in L3.3 with no significant effect of PEG-ADI, either alone or in combination with gemcitabine, D) MIA-PaCa-2 cells treated with PEG-ADI, gemcitabine or the combination with immunoblotting for caspase 3 following gemcitabine, PEG-ADI or the combination with notation of cleaved caspase 3, E) Effect of PEG-ADI, gemcitabine or the combination on Annexin V staining of MIA-PaCa-2 cells.