Figure 3
Regulation of RRM2 by E2F-1. A) Effect of increasing doses of gemcitabine on RRM2 levels in MIA-PaCa-2 cells, B) Effect of E2F-1 siRNA on E2F-1 protein levels in MIA-PaCa-2 cells, C) Effect of E2F-1 siRNA on E2F-1 transcription function in MIA-PaCa-2 cells determined by E2F-1-driven luciferase expression, D) Effect of E2F-1 siRNA on RRM2 levels following treatment with gemcitabine, PEG-ADI or the combination compared to the gemcitabine-mediated upregulation of RRM2, E) Effect of gemcitabine, PEG-ADI or the combination on E2F-1 transcription function in MIA-PaCa-2 cells determined by E2F-1-driven luciferase expression.