Figure 1

The expression of CXCR4 on intrahepatic cholangiocarcinoma (IHCC). (A) Immunohistochemistry staining of CXCR4 on IHCC tissues, ×200: a, c, e; ×400: b, d, f. negative control (a, b), low expression (c, d), high expression (e, f); (B) Kaplan-Meier survival analysis according to cytoplasmic CXCR4 expression in 122 patients with IHCC (log-rank test), P < 0.001 and the median survival time of the CXCR4 high and low expression groups was 20.0 (95% CI: 19.03-21.0) and 31.0 (95% CI: 25.6-36.4) months, respectively; (C) Western blot analysis revealed the CXCR4 expression for 4 IHCC cells (HCCC-9810, HuCCT1, RBE and Huh28). β-actin was used as a loading control.