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Figure 2 | Journal of Experimental & Clinical Cancer Research

Figure 2

From: Blockade of CXCL12/CXCR4 signaling inhibits intrahepatic cholangiocarcinoma progression and metastasis via inactivation of canonical Wnt pathway

Figure 2

Establishment of stable CXCR4 knockdown. (A) QT–PCR analysis of CXCR4 expression after the transfection of different CXCR4 shRNA expression vectors: shCXCR4-1, shCXCR4-2, shCXCR4-3, shCXCR4-NC (negative control) and HuCCT1, ***P < 0.001 compared with shCXCR4-NC cells; (B) Western blot with CXCR4 antibody of different transfected IHCC cells. β-actin was used as a loading control; (C) Western blot showed the different expressions2 of CXCR4 and phosphorylated CXCR4 on Serine 339 (P339-CXCR4), together with the p-CXCR4/total CXCR4 ratio (*P < 0.05) on transfected cells. β-actin was used as a loading control.

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