Figure 3

The influence of CXCR4 knockdown on the cell phenotype. (A) CCK-8 assay was analyzed for shCXCR4, shCXCR4-NC, HuCCT1 and CXCL12 (100 ng/ml) stimulated shCXCR4-NC on days 1, 2, 3, 4, and 5; (B) Distribution of cell cycle phases was demonstrated by flow cytometric analysis of shCXCR4 and shCXCR4-NC cells; (C) Colony assay was assessed to evaluate the cell colony formation ability. The count number of the colony was shown in the diagram; (D) The tumor formation ability of shCXCR4 and shCXCR4-NC cells in vivo. *P < 0.05, ** P < 0.01, ***P < 0.001 compared with shCXCR4-NC cells.