Brachyury enhances metastatic potential of HCC cells. (A) The invasive properties of the cells were analyzed in by an invasion assay using a Matrigelcoated Boyden chamber. Migrated cells were plotted as the average number of cells per field of view from 3 different experiments, as described in Materials and Methods. (B) The cell migration rate between SMMC-7721/Brachyury and SMMC-7721/GFP cells was compared by wound-healing assay. Microscopic observation was recorded at 0 and 24 hours after scratching the cell layer. (C) Representative view of lung tissue sections from each group are shown (H&E stain; magnification × 100). The number of lung metastatic foci in each group (n = 10) of HepG2/Brachyury or HepG2/GFP xenografted mice was calculated microscopically 6 weeks after tail vein injection. *p < 0.05.