Figure 4

Brachyury promotes EMT in HCC cells. (A) HepG2/ Brachyury and control cells were cultured on Poly-L-Lysine coated glass coverslips for 24 hours followed by immunofluorescence assay. (B) Relative expression of E-cadherin, γ-catenin, and Vimentin in HepG2/ Brachyury cells compared to control cells. (C) Representative view of Western blot assay of E-cadherin and Vimentin in tumor tissue (T) and paired peri-tumor tissue (N) from HCC patients with up-regulated Brachyury in tumor. (D) Scatter plot with fitted values intervals for T/NT (ratio of tumor tissue and paired peri-tumor tissue) expression of Bachyury and Vimentin. (E) Scatter plot with fitted values intervals for T/NT expression of Bachyury and E-cadherin.