Growth inhibitory effects of HATi II in glioma cell lines. (A-D) Cell viability assay. Glioma cells cultured in 96-well plates were treated with different concentrations of HATi II or DMSO for 48 h, and cell viability was determined using the CCK-8 assay. Cell proliferation was calculated as a percentage of the DMSO-treated control cells; IC50 values were derived by plotting the proliferation values on a logarithmic curve. The experiments were repeated three times. (E) Phase-contrast microscopy of U251, HS683, U87 and SHG44 cells treated with HATi II for 24 or 48 h (×100).