Figure 5

Bioinformatic analysis of the differentially expressed genes in HATi II- treated U251 cells. Gene ontology enrichment analysis provides a controlled vocabulary to describe the attributes of differentially expressed transcripts in all organisms (p-values ≤ 0.05 are recommended). (A) Upregulated genes and (B) downregulated genes in HATi II-treated U251 cells. Pathway analysis is a functional analysis that maps genes to KEGG pathways. The top five upregulated pathways (C) and top five downregulated pathways (D) in HATi II-treated U251 cells are listed. The “p53 signaling pathway”, which is associated with apoptosis, was activated in HATi II-treated U251 cells. (E) Confirmation of the microarray results. The expression of p53 signaling pathway-related genes in HATi II-treated U251, HS683, U87 and SHG44 cells was validated by quantitative real-time PCR. The columns represent the log-transformed median fold changes in expression relative to DMSO-treated cells.