Figure 3
CFZ treatment reduces cell viability through induction of apoptosis and can induce autophagy. Cells were left untreated or exposed to CFZ or CDDP at the 48 hour IC50 dose. A) Viability at 48, 72, and 96 hours was determined by direct cell counting after trypan blue staining. Mean ± SD are shown (n = 4). B) Detection of PARP, cleaved PARP, cleaved caspase-3, and C) LC3B by immunoblot in total extracts of cells harvested at 24, 48, and 72 hours. Boxed areas highlight the level of LC3B-II in CFZ treated cells which is a marker of autophagy. α-tubulin is shown as a loading control.