Figure 4

In vivo responses of SHP77 xenografts to CFZ and CDDP. Groups of scid mice (n = 8) were treated as detailed in the Methods section. (A) Mean tumor growth curves of SHP77 xenografts. The x-axis depicts days post tumor cell injection. Drug treatment was initiated on day 20 as indicated by arrow. (B, C, D) Kaplan-Meier survival curves of SHP77 tumor bearing mice for control and CFZ treated, control and CDDP treated, and control and CFZ/CDDP combination treated groups. The log-rank (Mantel-Cox) test was performed to evaluate the differences in survival between groups. A significant difference (p < 0.05) in survival was found between the control and CFZ treated groups. (E) SHP77 xenograft groups (n = 4) were treated two consecutive days with either vehicle or CFZ (5 mg/kg). Tumors were harvested 48 hours after the second dose and fixed sections were processed for immunohistochemical analysis. Representative photographs of immunoreactivity for cleaved caspase-3 at magnification of 60× and 400× are shown. Scale bar on 400× images = 50 μm. The percent of cells with cleaved caspase-3 is significantly higher in the CFZ treated tumors versus the vehicle treated tumors (*p < 0.05 using Student’s t-test).