Figure 5

Impact of depleting endogenous YB-1 on PC-3 survival after CPT treatment. A. Downregulation of YB-1 expression by two different YB-1 siRNAs. Control siRNA (50 pmol) or YB-1 siRNA were transfected into PC-3 cells, and whole-cell lysates (50 μg) were subjected to SDS–PAGE. Transferred proteins were probed with anti-YB-1 and anti-LaminB1 antibodies. B. Knockdown of YB-1 renders cells resistant to CPT. PC-3 cells were treated with 50 pmol of YB-1 siRNA or 50 pmol of control siRNA for 24 h, and then exposed to various concentrations of CPT for 48 h. Cells were stained with TetraColor ONE and their absorbance was measured at 450 nm. All values were the mean of least three independent experiments.