Figure 1

miR-301a was upregulated in lymph node metastatic CRC tissues and highly metastatic CRC cells. (A) qRT-PCR analysis of 48 pairs of CRC samples and matched adjacent normal mucosa; U6 small nuclear RNA was used as an internal control. There was no significant difference in miR-301a expression between CRC tissues and their adjacent non-tumor tissues. (B) miR-301a expression levels in primary CRCs with lymph node metastasis were much higher than that of CRCs without lymph node metastasis. (C) The migratory potential was examined by migration assays. (D) Relative expression of miR-301a in 5 CRC cell lines by qRT-PCR with U6 small nuclear RNA as an internal control. Data were representedas means ± SD from 3 independent experiments. ^#, P > 0.05; *, P < 0.05; **, P < 0.01.