Figure 2

miR-301a promoted CRC cell migration and invasion in vitro . (A) Expression levels of miR-301a in SW620 cells after transfection with inhibitors of miR-301a, as determined by qRT-PCR. U6 small nuclear RNA was used as an internal control. (B) Expression levels of miR-301a in SW480 cells after transfection with synthetic miR-301a mimics, as determined by qRT-PCR. U6 small nuclear RNA was used as an internal control. (C) Downregulation of miR-301a with its inhibitors impeded cell migration and invasion of SW620 cells. (D) Overexpression of miR-301a significantly promoted cell migration and invasion in SW480 cells. (E) miR-301a inhibitors significantly inhibited SW620 cell motility as shown in wound healing assay. (F) miR-301a mimics significantly promoted SW480 cell migration in wound healing assay. Data were representedas means ± SD from 3 independent experiments. **, P < 0.01.