Figure 4
From: MicroRNA-301a promotes migration and invasion by targeting TGFBR2 in human colorectal cancer
TGFBR2 was a direct target gene of miR-301a. (A) qRT-PCR showed that neither miR-301a mimics nor anti-miR-301a changed the mRNA level of TGFBR2. (B) anti-miR-301a in SW620 cells suppressed, while miR-301a mimics in SW480 cells increased the protein expression of TGFBR2. (C) Immunohistochemical staining of TGFBR2 in tumor sections. Expression of TGFBR2 was evaluated in tumors formed by SW620/LV-anti-miR-301a cells. (D) Schematic picture of two miR-301a binding sites and the corresponding mutants on TGFBR2 3’-UTR. (E) Luciferase activity assay identified the binding sites of miR-301a. Data were representedas means ± SD from 3 independent experiments. Magnification, ×200. Scale bars, 100 μm. *, P < 0.05; **, P < 0.01.