Effect of Pim-1 siRNA on Pim-1 expression level and cell viability in SACC cells. A. Real time PCR results displayed the Pim-1 mRNA expression in both cell lines. B. Western blot showed the inhibition effect of Pim-1 siRNA on Pim-1 protein levels in both cell lines. The expression of β-actin was used as a quantitative control. C. CCK-8 assay results of SACC-83 cell after transfected with Pim-1 siRNA for 48 h and 72 h. D. CCK-8 assay results of SACC-LM cell after transfected with Pim-1 siRNA for 48 h and 72 h. Data were showed as mean ± SD. p < 0.05, *Pim-1 siRNA group compared with control siRNA group.