Figure 1

mir-34a was downregulated in bladder cancer and circulatingmiR-34a levels are reduced in bladder cancer. A, relative expression of mir-34a expression levels were evaluated by qPCR in bladder cancer.U6 small nuclear RNA was used as an internal control. B, relative expression of circulating mir-34a expression levels were evaluated by qPCR in bladder cancer. U6 small nuclear RNA was used as an internal control. C, The expression of mir-34a in blood stream positively correlates with the expression in bladder tissues (R2 = 0.3036 P = 0.0411, Pearson’s correlation). D, relative expression of CD44 expression levels were evaluated by qPCR in bladder carcinoma cell lines. C, miR-34a expression after mimics transcription was detected by qPCR. E, MiR-34a inversely correlates with CD44 expression (R2 = 0.5988 P = 0.0412, Pearson’s correlation). F, relative expression of CD44v6 expression levels were evaluated by qPCR in bladder carcinoma cell lines. G, CD44v expression pattern in human bladder cancer cell lines and bladder cancer tissues. Data are plotted as the mean ± SEM of 3 independent experiments. **, P < 0.01.