Figure 4

mir-34a targets CD44 in bladder cancer cells and the validation of transfection/infection. A and B, mir-34a expression was detected by qPCR after infection of miR-34a lentivirus and corresponding negative control in 5637 and T24 for 4 days. C and D, mir-34a expression was detected by qPCR after infection of miR-34a mimics or inhibitor and corresponding negative control in 5637 and T24 for 48 hours. CD44 protein expression was inhibited in miR-34a transfected bladder carcinoma cells. E, CD44 expression was detected by western blot after overexpression of miR-34a and corresponding negative control i 5637 and T24. F and G, The seed regions of the miR-34a target sites in CD44 and the luciferase activity assay. *, P < 0.05; **, P < 0.01.