Figure 2

Effect of DAL-1 overexpression on morphology and EMT markers in A549 cells. Cells were stably transfected with pcDNA3-DAL-1 or pcDNA3 plasmid respectively. A, Morphological changes in DAL-1 overexpression cells, from spindle-like mesenchymal morphology into epithelial morphology, by manifesting an increased cell-to-cell adhesion; B, Q-PCR analysis of the levels of EMT related markers after DAL-1stably transfected with pcDNA3-DAL-1 plasmid; The levels of E-cadherin and β-catenin mRNAs were higher in the cells treated with the pcDNA3-DAL-1 plasmid than in the pcDNA3 treated cells (mock) or A549 cells (blank), but the level of Vimentin and N-cadherin mRNAs were lower. The data are presented as the mean ± SD. n = 3, *P <0.05,#P <0.01. C, Western blot analysis of DAL-1 and EMT marker protein expression levels in A549 cells. When the cells were transfected with the pcDNA3-DAL-1, the protein levels of E-cadherin and β-catenin increased while protein levels of Vimentin N-cadherin were down-regulated.