Figure 6

Overexpression of EBF3 inhibited proliferation and induced apoptosis in leukemia cells. (A) Western blot analysis of EBF3 expression in EBF3 transfected leukemia cells. Transfection with EBF3 lentivirus PLVX-EBF3 significantly upregulated expression of EBF3 in AML cells compared with mock-transfected cells. An expression level of cleaved PARP, a marker of apoptosis, was analyzed by Western blotting. (B) CCK-8 assays show that transfection with EBF3 lentivirus inhibits proliferation in HL-60 and MV4-11 cells compared with mock-transfected cells. (C) The number of cells displaying apoptotic features is higher in the HL-60 and MV4-11 cells transfected with PLVX-EBF3 compared with the mock-transfected cells. (D) Quantification shows that the proportion of apoptotic cells in the EBF3-overexpressing cells (PLVX-EBF3) was significantly greater than the vector control group (PLVX-Ve). **P < 0.01.