Table 1 Primer sequences for MSP
Gene | Sense 5'→3' | Antisense 5'→3’ | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
ABCC6 M | GGCGTTCGGGGAGTT | CGACCTCGACCCGATAAT | 57 | 247 |
ABCC6 U | AGGTGTTTGGGGAGTTGG | TCTCAACCTCAACCCAATAATC | 58 | 244 |
BRCA1 M | TCGTGGTAACGGAAAAGCGC | AAATCTCAACGAACTCACGCC | 58 | 75 |
BRCA1 U | TTGGTTTTTGTGGTAATGGAAAAGTGT | CAAAAAATCTCAACAAACTCACACCA | 56 | 86 |
CDH1 M | GTGGGCGGGTCGTTAGTTTC | CTCACAAATACTTTACAATTCCGACG | 57 | 172 |
CDH1 U | GGTGGGTGGGTTGTTAGTTTTGT | AACTCACAAATCTTTACAATTCCAAC | 59 | 172 |
GDF15 M | CGGCGGTTATTTGTATTTGC | AACGATCGTATCACGTCCC | 60 | 132 |
GDF15 U | ATTTGGTGGTTATTTGTATTTGT | AACAATCATATCACATCCCACA | 57 | 135 |
HSPA2 M | TAAGAATCGGGAATTGGGC | AATCGATACCGATAACCGAA | 58 | 172 |
HSPA2 U | TTATAAGAATTGGGAATTGGGT | AAATCAATACCAATAACCAAA | 55 | 176 |
RASSF1A M | GGGTTTTGCGAGAGCGCG | GCTAACAAACGCGAACCG | 64 | 169 |
RASSF1A U | GGTTTTGTGAGAGTGTGTTTAG | CACTAACAAACACAAACCAAAC | 59 | 169 |
SALL3 M | GTTCGCGTAGTCGTCGTC | TACTCGAAAACCCCGTCA | 57 | 203 |
SALL3 U | GTGGTTTGTGTAGTTGTTGTTGTT | CCCAACCCTCACCATACTC | 57 | 220 |
THBS1 M | TGCGAGCGTTTTTTTAAATGC | TAAACTCGCAAACCAACTCG | 62 | 74 |
THBS1 U | GTTTGGTTGTTGTTTATTGGTTG | CCTAAACTCACAAACCAACTCA | 62 | 115 |
TMEFF2 M | GAAGAGGGGCGTTAGTTC | ACGCTAACCCGAATAAAACT | 57 | 151 |
TMEFF2 U | GGAAGAGGGGTGTTAGTT | AACACTAACCCAAATAAAACT | 55 | 153 |
VIM M | TTATAAAAATAGCGTTTTCGGC | ATAACGCGAACTAACTCCCG | 59 | 143 |
VIM U | GGGTTATAAAAATAGTGTTTTTGGT | ACAATAACACAAACTAACTCCCA | 56 | 149 |