TFCP2 promotes human HCC cell growth,
. (A) Real-time PCR and (B) western blotting for TFCP2 expression in human HCC cell lines, Red and blue bars represent high and low TFCP2 expression in HCC cells, respectively in A. β-actin as a loading control. (C, D) Western blotting of TFCP2 changes in HCC cells after siRNA transfection for 48 h or lentivirus infection. (C) Decreased TFCP2 protein in HepG2 and BEL-7402 cells transfected with siTFCP2 compared with negative control (siNC), (D) Increased TFCP2-HA fusion protein in Hep3B and SK-HEP-1 cells with lentivirus infection compared with negative control (Lenti-NC). (E, F) Cell proliferation assays in HCC cells with altered TFCP2 expression. The cell viability was determined by the CCK-8 assay in 96-well plates. All determinations were performed at least in seven replicates in three independent experiments. Values represent O.D. values mean ± SD (*P < 0.05, **P < 0.01; upper, HepG2 or SK-HEP1, lower, BEL-7402 or Hep3B). (E) HepG2 and BEL-7402 cells transfected with siRNA (F) Hep3B and SK-HEP-1 cells with lentivirus infection. (G, H) Colony formation assays in HCC cells with altered TFCP2 expression in cell culture plates. Representative photographs of colony formation from different cell lines are shown in the upper panels. The colony formation rate is shown in the lower panels (calculated by dividing the colony numbers by the plated cells). Data were obtained from three independent experiments. (G) HepG2 and BEL-7402 cells transfected with siRNA for 24 h and then used for colony assays, (H) Hep3B and SK-HEP-1 cells with lentivirus infection (**P < 0.01).