The effect of TFCP2 on cell migration and invasion. The TFCP2 knockdown in HepG2 significantly inhibited the migration and invasion of HCC cells compared with controls, HepG2 transfected with siRNA for 24 h and then used for miagration and invasion. Whereas TFCP2 overexpression in SK-HEP-1 promoted migration and invasion. The migration and invasion assays were assessed at 24 and 48 h, respectively. Representative fields of invaded cells from independent groups are shown in the upper panels. Quantitative analysis of invaded cells is shown in the lower panels. The data are presented as mean ± SD of three different experiments (*P < 0.01).