Figure 6
TFCP2 promotes migration/invasion through up-regulating FN1 and down-regulating TJP1. (A) FN1 inhibition partially reverses the aggressive effect by TFCP2 overexpression in SK-HEP-1 and Hep3B cells. Transient transfection with FN1 siRNA results in a significant reduction in migratory and invasive behaviors analyzed by migration and invasion assays (*, P < 0.05 compared with SiNC). Cells infected by TFCP2 vectors and transiently transfected with FN1 siRNA showed no increase in aggression by TFCP2 overexpression (**, P > 0.05 compared with cells with Lenti-NC + SiNC). (B) SiTJP1 could also rescue, at least in part, the aggressive effect of TFCP2 knockdown in HepG2 and BEL-7402 cells. Transient transfection with TJP1 siRNA results in a significant increase in migratory and invasive behaviors analyzed by migration and invasion assays (*, P < 0.05 compared with SiNC). Cells transfected by si-TFCP2 and TJP1 siRNA showed no reduction in aggression by si-TFCP2 (**, P > 0.05 compared with cells with SiNC + SiNC).