Figure 1

Relative MALAT1 expression in cell lines and tissues assessed by qRT-PCR and its clinical significance. (A) Expression level of MALAT1 in ESCC cell lines (EC109, EC9706, KYSE450, and KYSE150) compared with that of the normal epithelial cell line Het-1A, data was presented as expression fold-change relative to Het-1A. (B) Over-expression of MALAT1 was detected in 46.2% of ESCC tissues. Relative expression of MALAT1 was detected in 54 pairs of clinical samples and normalized to GADPH; Data was presented as fold-change in tumor tissues relative to normal tissues. The red column was defined as over-expression. (C) Difference in expression levels of MALAT1 between cancerous and normal esophageal tissues. The statistical differences between the two groups were analyzed by paired Student’s t-test. (D) MALAT1 expression was significantly higher in patients at advanced pathological stages; one-way ANOVA analysis was performed to calculate the statistical difference. (E, F) MALAT1 expression was significantly higher in samples with larger tumor size and lymph node metastasis. The statistical differences between the two groups were analyzed using unpaired Student’s t-test. *P < 0.05, **P < 0.01.