Figure 2

Knockdown of MALAT1 inhibits esophageal cancer cells growth in vitro and in vivo . (A) Expression level of MALAT1 in ESCC cell lines following treatment with si-MALAT1 or si-NC. si1 and si2 indicate the two small interfering RNA used in this study. (B) Knockdown of MALAT1 suppressed ESCC growth in vitro. CCK8 assays were performed to determine the proliferation of ESCC cells transfected with siMALAT1 and si-NC. (C) Colony-forming growth assays were also performed to determine the proliferation of EC109 and EC9706 cells. The colonies were counted and captured. (D) Representative image of tumor growth on nude mice. (E) Effects of MALAT1 knockdown on tumor growth in vivo, tumors developed from si-MALAT1 stably transfected EC109 cells showed smaller tumor volume and weights. *0.05, **P < 0.01.