E-cadherin expression on lung dendritic cell in terms of lung tumour immunity, adoptive immunity (C57BL6), innate immunity (Rag1 KO) and CD40-mediated immune response (CD40/Rag1 ko). (A) Lung tissues were harvested from the Lewis lung cancer model, C57/BL6 mice, Rag1 KO mice and the anti-CD40 model, respectively. The green fluorescence represents CD11c, and red represents E-cadherin. (B) The phenotype of inflammatory E-cadherin + DCs in lung and spleen cell suspensions. The spleen and lung cells were harvested from the anti-CD40 model. For the lung cell test, E-cadherin and CD11c expression was analysed in sorted CD11chigh cells prepared from a lung single-cell suspension (sorted using a BD FACSAria III flow cytometer). Staining for CD11b, CD4, CD103 and E-cadherin and were gated on E-cadherin+/−CD11chigh cells from lung cells. For the spleen cell test, E-cadherin and CD11c expression was examined in a spleen single-cell suspension. Staining for CD4, CD103 and E-cadherin gated on E-cadherin+/−CD11chigh cells to analyse the expression of CD103 and CD4. Each experiment was performed in triplicate.