Relationship between inflammatory E-cadherin + DCs and Th1 and Th2 cells. (A) The polarisation of Th1 cells with the percentage of CD4 + IFN-γ + T cells and the polarisation of Th2 cells with percentage of CD4 + IL-4+ T cells. Percentage of IFN-γ + CD4+ cells and IL-4 + CD4+ cells from the E-cadherin + DCs and E-cadherin- DCs groups, according to the gating of CD4+ T cells. (B) Secretion of cytokines from Th1 and Th2 cells. Cytokine secretion was measured in the supernatant using the FlowCytomix kit (eBioscience). The supernatant was harvested at 72 h and 96 h and then analysed for Th1 (IFN-γ) and Th2 (IL-4, IL-5, IL-6, IL-13). Each experiment was performed in triplicate. The asterisk indicates a significant difference between the two test groups, as analysed by Student’s t-test. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).